Both Resonant and Galvo versions of SciScan are now compliant with the Open Microscopy Environment (OME) XML Standard. Any TIFF saved from either version of the software will include OME compliant XML metadata while raw files will have a separate XML file saved along-side them.
If a recording is cancelled, the TIFF tag may include incorrect file size information, however a correct version of the XML metadata should be saved alongside it.
Notes on Use
Generation of the XML metadata is taken care of by the OME_coMD.vi optional module and needs to be activated in the .ini file before operation. Once enabled it can be accessed through the plugins menu.
There are three types of settings that are recorded in the metadata; Experiment/Experimenter Settings, Hardware Settings and Imaging Parameters.
Experiment and Experimenter settings fields are edited through the OME GUI accessed from the plugins menu. The fields provided allow the experimenter to fill in information about his/herself, the experiment, the sample, the objective used and the fluorophores that were used. Close the window to confirm changes to the metadata.
Parameters specific to the image being recorded (zoom, pixel size, resolution, number of frames ect…) are read directly from SciScan and written into the metadata.
Hardware settings are specific to the imaging equipment being used and typically do not change very often. Hardware settings include information such as the make and model of detectors, light sources and the microscope. Typically these values do not change between experiments or experimenters so are kept constant.
To edit these values the default XML file must be edited. The XML template used to generate the metadata for each file is found in Plugins/OME Module/OME Default.XML.
Hardware settings can be changed by directly editing this XML document using either text editor or code editors such as Notepad++ ( https://notepad-plus-plus.org/ ). Save the document after and any changes made will be reflected in the metadata of the next file saved.