The first four tabs located below the image display window are used to swap between imaging channels. Selecting a channel brings up the Image from the relevant PMT. Which channels are currently being used can be set on the General Settings Tab. See the Knowledge Base articles on the ROI Monitor and RGB Overlay for information on other display tabs.
When scanning is started, the image will be displayed in the image display window at its native resolution. you can enlarge the image simply by clicking in the display area. To zoom back out hold the shift key and click again.
To automatically scale the image to full the display area press the reset zoom button found to the top right of the display pane.
Please Note: Digitally zooming in the image pane is independent from the ZOOM control found in the 2D Frame Tab.
The blue bar and counter (Labelled “O” in the GUI overview) indicates the current progression through the number of frames to acquire (set in the 2D Frame Tab and will loop indefinitely if in Focus mode).
A grid can be overlaid over the image display window. Adjusting the slider indicated below can adjust the number of cells included in the grid. This can be adjusted from 1×1 (single cell with no additional grid overlay shown, such as in the above image) to a 20×20 grid overlay. The size of each cell will be calculated from the field of view settings and will be displayed next to the grid slider control.
|A||Grid Overlay UI Slider||The slider can be used to adjust grid overlay from 1x1 cells to 20x20 cells|
|B||Grid Cell Size||Cell size is calculated from field of view settings and displayed|
|C||Grid Overlay||Grid is overlaid on current image display window|
An example of a grid overlay with 6×6 cells can be seen below
The grid overlay feature is also available in the position save module and can be used to help align the current field of view with a reference image for cases where the Red/Blue overlay feature isn’t enough. For more information on using the Position Save Module please see the Position Save Article.
The histogram tab at the bottom of the imaging plane is used to adjust the displayed image. Each channel is given its own tab for independent adjustments.
|A||display palette||Allows selection between different display palettes|
|B||avg of||Allows you to display the image with a moving average, group average or no average applied, useful for imaging weak signals|
|C||avg type||Defines the number of frames being averaged if avg type is set|
|D||display every n-th frame||Allows you to change the display to every n-th frame to reduce load on the processor. If the raw data que between the FPGA and the image display increases due to processor load, this will automatically increase until the point that the raw que is no longer growing.|
|E||histogram||The histogram itself has an auto scale feature which will automatically adjust the image based on the incoming signal; if disabled you can manually adjust the range by sliding the two bars or adjusting the two numeric controls. A representation of the grey levels appears in the chart to the right.
|F||histogram tabs||switches between histograms of available channels|
This drop-down menu gives you the option of choosing from the following display pallets;
Standard – displays the image with no display palette, just greyscale.
High Clipping Red – Displays the image with saturated pixels showing up red on the display.
Low Clipping Blue – Displays the image with low saturated pixels displaying as blue.
High and low Clipping – Combines both the high clipping red and low clipping blue palettes