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The latest version of the software can be downloaded below.

Full documentation, as well as installation instructions, can be found in the KnowledgeBase section of this website.

Release VersionNotesDownload
SciScan 1.4• Windows 10 compatibility - SciScan1.4 now runs on LabVIEW 2018 and is fully compatible with the newest Windows 10 version.

• Piezo Z Positioning (All systems) - The user has the option to define the Z-volume using the Piezo actuator instead of the stage. This is useful in applications that require an accuracy higher than what is achievable using the stage.

• Piezo Inertia Compensation (All systems) - For fast volume scans, the actual Piezo position could lag the Pockels cell waveform due to mechanical inertia. This can be compensated by shifting the waveform in time.

• New Overlay Option for Position Save Module (All systems) - The Position Save Module now has the option of a Red/Green and Red/Blue overlay.

• Histogram (Galvo systems) - The histogram display now shows the unsigned 16-bit pixel values (after applying the necessary offset to acquired 16 data). The histogram values now reflect the saved data.

• 16-bit Scaling (Galvo systems) - With SciScan1.4 there is now an option to scale 12-bit data acquisition on Galvo systems to 16 bits using a scalar multiplication factor.

• Line Clock (Galvo systems) - The trigger tab for galvo scanning now has the option of the Line Clock feature via PFI12. It can be accessed from the trigger tab by selecting ‘Line Acq Clk (PFI12)’ and works for galvo scans with a fill fraction below 100%.

• Mirror Warmup feature (Resonant systems) - The X (resonant) scan mirror can be started before the actual acquisition so that the mirror frequency stabilises. This is useful for long acquisitions which could be affected by mirror frequency drift over time.

• Bug fixes - Performance improvements and optimisation of the RGB overlay / Bug fixes for z-stack tasks running in Macro / The display palette (Red-Blue clipping) has been optimised for performance

• Software requirements

a. SciScan1.4 for Win7 requires:
LabVIEW2012 Version
b. SciScan1.4 for Win10 requires:
LabVIEW2018 Full Development System (64bit) (NI Part number: 776670-35); LabVIEW Vision Development module 2018 (NI Part number: 777859-35); Visual Studio Express for Windows (2012); LibTIFF
SciScan1.4 (Win 10, LabVIEW2018)

SciScan1.4 (Win 7, LabVIEW2012)

SciScan1.4 Release Notes
SciScan 1.3• Multi-Scanner oriented – the software is now started from a VI named SciScan HyperScope. This provides access to a light path selector, pre-populated according to the present hardware configuration.

• Photostimulation (Galvo systems) - This module allows the use of the HyperScope’s second beam path for multiphoton photostimulation. Photostimulation can run on its own or concomitantly with imaging.

• Calibration Module (HyperScope dual path) - a calibration module has been created to register the photostimulation path to the imaging path. Doing so will allow simultaneous imaging and photostimulation through the photostimulation module.

• New optional plugins are now available: artificial motor, KR module, replay logger, Piezo Z-positioning module

• SciScript “Generic” macro command now accessible - Any GFG variable (e.g. Laser.Power) can now be used and modified in the macro module for advanced scripting and generation of new macros.
SciScan1.3

SciScan1.3 Release Notes
SciScan 1.2• Uncaging Module – The uncaging module is designed to allow users to carry out Multiphoton, single-point, uncaging or photo activation experiments while recording the result using electrophysiology equipment.

• Tiling Pre-set for SciScript – A pre-set has been added to SciScript to allow systematic image capture for regions larger than the maximum FOV of the objective. Tile pre-sets are available for both 2D and 3D acquisitions.

• Advanced Trigger Output – SciScan’s trigger interface has been expanded to output user specifiable pulse patterns to aid in triggering of external devices and synchronising equipment with SciScan.

• Zoom and Move to ROI – The Zoom and Move to ROI function allows you to mark either a point in your FOV and the move it to the centre of the field by moving the XY stage, or allows you to select a rectangular region of interest and zoom to it easily.

• Arbitrary Piezo Scale Factor – Previously, scale factors for either 400um travel piezo drives, or 100um travel piezo drives were available. The Scale Factor can now be set manually through the .ini file to provide scaled outputs for other devices.
SciScan1.2


SciScan1.2 Release Notes
SciScan 1.1.5• Changes to protocol notes- Added a “Save” button to manually save notes into the root folder. (n.b: Protocol notes are always saved automatically alongside each recording, but can now also be saved manually.) Time-stamp button function has been altered to include the current frame count along with the time-stamp.

• Grid Overlay Feature- Added the option to overlay the displayed image with an adjustable size grid in both normal image display and in the position save module.

• For bug fixes, please see release notes
SciScan1.1.5 Release Notes
SciScan 1.1• Start Delay Look-up-table – Automatically records the last used start delay value for a set of scan parameters and re-uses this value when these parameters are used again.

• Open Microscopy Environment Compatibility - Allows users to save images with OME compliant metadata and add additional content to the metadata if required.

• 16-Bit Multi-TIFFs for Resonant Scanning – Can now save resonant scanning data as 16-bit Multi-tiff

• Objective selection for resonant scanning system – A drop down has been added to save and select different objective configurations.

• SciScript Pre-sets – A Slow Z-Stack preset has been added to SciScript, more presets will follow.

• Stand Alone line shift correction - A stand-alone VI (VI Name) has been included in the Extras folder in the SciScan Project. The VI allows re-aligning of raw images that were recorded with an incorrect start delay setting.

• Python and Matlab Interface Added to Zip File – Communicate with SciScan through ActiveX Interface

• For minor changes and bug fixes please see release notes
SciScan1.1 Release Notes
SciScan 1.0New release of SciScan 1.0 with small bug fix
SciScan 1.0• Frame rate up to ~50fps with the galvo scan head (200x50 pixels)

• Frame rate up to ~248fps with the resonant scan head (1024x64 pixel)

• TTL OUT channels (sample clock, pixel clock, line clock, frame clock) for accurate synchronization with external equipment

• TTL IN for triggering a recording from external equipment

• Up to 4 PMT channels

• Integrated intensity control (using a Pockels cell or a motorised half-wave plate)

• Fast volume scanning using a piezo objective positioner

• Standard scan modes include: frame scan, Z-stacks, line scan

• ROI Monitoring

• Position Save Module for chronic in-vivo imaging enables users to save multiple recording locations and easily find the same locations again days or weeks later

• SciScript - intuitive user interface for creating pre-programmed sequences and loops of frame scans, recordings